Journal: Neurochemical Research

Article Title: Morin Improves Cognitive Deficits in an in Vivo Model of Vascular Dementia by Modulating the N-methyl-D-aspartate Receptor Signaling Pathways

doi: 10.1007/s11064-026-04717-7

Figure Lengend Snippet: The impact of morin on the expression of synaptic plasticity-related proteins in the hippocampus of VaD rats. a Western blots; b the protein levels of SYP; c the protein levels of PSD-95; d the expression levels of SYP ; e the expression levels of PSD-95; f the levels of glutamate. Data are presented as mean ± SD. Statistical analysis was performed by one-way ANOVA with Tukey’s post-hoc test (data met assumptions of normality and homoscedasticity)/Kruskal-Wallis with Dunn’s test (data did not meet assumptions). * indicates a significant difference from the Sham group; # indicates a significant difference 2VO group; *# indicates a significant difference from both the Sham and 2VO groups, with a p-value of less than 0.05 considered statistically significant

Article Snippet: Primary antibodies, all diluted at 1:1000, were sourced from Santa Cruz Biotechnology and included the following: PSD-95 (sc-32290, Santa Cruz Biotechnology, Inc.), SYP (sc-17750, Santa Cruz Biotechnology, Inc.), and β-actin (sc-517582, Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Western Blot

Journal: Frontiers in cellular and infection microbiology

Article Title: Hydrogel-based 3D human iPSC-derived neuronal culture for the study of rabies virus infection.

doi: 10.3389/fcimb.2023.1215205

Figure Lengend Snippet: FIGURE 3 Impact of collagen-Matrigel on neuronal differentiation, maturation and synapse formation. (A) HiPSC-derived neurons were seeded in 2D monolayer, 3D collagen hydrogels and 3D collagen-Matrigel hydrogel blends at the density of 1×106 cells/ml. Cells were stained with b-III tubulin and Hoechst at day 7. Representative confocal micrographs of b-III tubulin-positive cells (red). Scale bars = 100 µm. The morphological parameters of neurons including (B) total neurite length, (C) average neurite length, and (D) maximum neurite length was quantified using NeuronJ plugin in ImageJ for three randomly selected fields of view (20x) for each experimental batch with approximately 150 cells per field of view. (E) A graph showing the percentage of MAP2-positive neurons calculated per fields of view (20x). Data are represented as mean ± SEM; n = 4-6. (F–I) HiPSC- derived neurons were seeded in 2D monolayer, 3D collagen hydrogels and 3D 1:1.25 mg/ml collagen-Matrigel hydrogel blends at the density of 1×106 cells/ml. Total RNA was isolated at indicated time points. Graphs present the fold change of b-III tubulin, MAP2, PSD95, and Synapsin1 gene expression by RT-qPCR. Fold-changes were expressed by normalizing to their respective control, 2D culture at day 7. Data are represented as mean ± SEM; n = 3. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; COL, Collagen; MAT, Matrigel.

Article Snippet: After the blocking step, appropriate primary antibody dilutions of rabbit a-MAP2 (Abcam; ab183830), rabbit a-b-III tubulin (Abcam; ab18207), rat a-CTIP2 (Abcam; ab 18465), mouse a-PSD95 (Novus Biologicals; nb300-556) and horse a-rabies antiserum were added and incubated overnight at 4°C.

Techniques: Derivative Assay, Staining, Isolation, Gene Expression, Quantitative RT-PCR, Control